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Multiple Comparisons/Post-Hoc Tests

In an analysis of variance, if the null hypothesis is rejected, i.e., at least two of the group means differ significantly, we still may not know which group means differ and how many group means differ from each other. The multiple comparison/post-hoc tests are designed to answer these questions. There are two types of comparison tests, i.e., simple comparisons and complex comparisons.

The α Level

Analysis Output

Multiple Comparisons Accompanying Analysis of Variance (ANOVA)

Tests That Can Be Performed With Equal Group Sizes:

Tests That Can Be Performed With Both Equal and Unequal Group Sizes:

The more conservative a test, the less powerful, and the lower the risk of making a Type I error (i.e., incorrectly deducing a difference, when in fact there is no significant difference). However, reducing the risk of making a Type I error increases the chance of making a type II error (i.e., incorrectly deducing no difference, when in fact there is a significant difference).

Scheffé test is commonly described as the most conservative of the unplanned tests:

Testing Independent Groups vs. a Given Control Group

Aabel provides the Dunnett test for pair-wise comparisons where all independent groups are compared with the same control group.

Example The response value ranges from four independent groups of subjects, where three groups have received an experimental treatment (E1, E2, E3), and a control group has received placebo, are compared in the box & whisker plot below. The corresponding one-way between-subjects ANOVA report indicates that at least two groups have means that differ significantly. The Dunnett test reveals that it is only the experimental group E1 that differs significantly from the control group.


Multiple Comparisons Accompanying Analysis of Covariance (ANCOVA)

The multiple comparisons/post-hoc tests accompanying the analysis of covariance are conducted on The Adjusted Group/Treatment Means, and include:

  • Tukey's HSD test
  • Scheffé test
  • The expressions employed to compute the test statistic for the tests accompanying ANCOVA are different from those used for tests accompanying ANOVA. In the former, computing adjusted group/treatment means determines what the scores on dependent variable would be if the groups did not differ on the covariate.