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Two-Way Within-Subjects (Repeated Measures) ANOVA
Two-way repeated measures ANOVA combines elements of two-factor between-subjects design and single-factor within-subjects design. That is, the variability is computed for the main effects of Factor A, the main effect of Factor B, and the interaction (AB), but the design requires three error terms, one for each effect.
Two-Way Repeated Measures ANOVA Output
The default output includes the two-way ANOVA table. The additional output depends on selected options, and can include interaction plots, the results for sphericity test and correction, and table reports for selected multiple comparisons tests.
The Default Output: One-Way ANOVA Table
This table displays:
- The source of variability (i) attributable to Factor A, (ii) attributable to Factor B, (iii) attributable to AB interaction, and (iv) attributable to error terms for each effect (i.e., A x Subjects, B x Subjects, AB x Subjects)
- The sum of the squared deviations from the mean (Sum of Squares); the degrees of freedom (df); the Mean Square for each of the variability components
- The two recommended F statistics: F+ (which is the preferred test statistic when omnibus sphericity is tenable), the Fx statistics (which can be used when omnibus sphericity is not tenable, but local sphericity condition is satisfied)
- The p+ value corresponding to F+, and the px value corresponding to Fx
- The partial omega squared test result (testing strength of association)

Interaction Plots
Interaction plots are a graphical display of the effect of one factor at each level of the other factor. With no or insignificant interaction, the lines are approximately parallel. The more the lines diverge from being parallel, the stronger the interaction.
The error bars displayed on the interaction plots can represent:
- Standard error of mean
- Standard Deviation
- Confidence Interval (including options of 90.0%, 95.0%, 97.5%, 99.0%)

Sphericity Evaluation (Locally Best Invariant Test)
Greenhouse & Geisser and Huynd & Feldt Corrections
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Multiple Comparisons/Post-Hoc Tests
Simple comparisons (also know as pair-wise comparisons) accompanying two-way within-subjects ANOVA include:
The example below is the Fisher's LSD test result for the two-way within-subjects ANOVA example illustrated above. For more information regarding multiple comparisons, click here. ![]() Supported Worksheet LayoutThis ANOVA design requires (i) two categorical columns for storing levels of the design within-subjects factors (i.e., Factor A with p >= 2 levels, and Factor B with q >=2 levels), and (ii) a numeric column for storing the experimental scores (response values) from all of the pq experimental conditions (see the right-hand side image below). In this layout, a subject occurs in multiple worksheet rows: hence, it is required to have a data column (numeric or categorical) with the subject ID. |
Below, is an example of the data layout used for two-way repeated measures ANOVA:
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